Journal: Cancers

Article Title: Preclinical Evaluation of the Novel Small-Molecule MSI-N1014 for Treating Drug-Resistant Colon Cancer via the LGR5/β-catenin/miR-142-3p Network and Reducing Cancer-Associated Fibroblast Transformation

doi: 10.3390/cancers12061590

Figure Lengend Snippet: Cancer-associated fibroblasts (CAFs) increased the oncogenic properties of colon cancer cells. ( A ) Insert illustrates the co-culture system of DLD1 and HCT116 colorectal cancer (CRC) cells with Normal fibroblasts (NF) and CAFs. Cell viability assay showed increased 5-fluorouracil (5-FU) resistance in CAF-educated DLD1 and HCT116 cells, compared to their naïve counterparts. Flow cytometric analysis of DLD1 and HCT116 cells co-cultured with CAFs. Increased CD44 + cell population ( B ) and side population ( C ) in both DLD1 and HCT116 cells, 48 h post CAF culture. Enhanced colony-forming ( D ), migratory ( E ), and tumor sphere-generating ( F ) abilities in both CRC cell lines post CAF co-culture. ** p < 0.01, *** p < 0.001.

Article Snippet: The DLD1 and HCT116 human colon cancer cell lines and normal fibroblasts (NFs, ATCC® PCS-201-018) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and were cultured according to the vendor’s recommended conditions.

Techniques: Co-Culture Assay, Viability Assay, Cell Culture

Journal: BioMed Research International

Article Title: Astaxanthin Enhances Gingival Wound Healing following High Glucose-Induced Oxidative Stress

doi: 10.1155/2022/4043105

Figure Lengend Snippet: Effects of ASTX on ROS levels induced by hyperglycemia in human gingival fibroblasts were determined by confocal microscopy. Representative images of DCFDA from 5-, 25-, and 50 mM glucose and ASTX-treated groups are given. To monitor the ROS levels, DCFDA loaded cells were calibrated with 100 μ M H 2 O 2 . Changes in total fluorescent intensity in LG group were higher than HG groups, as well as ASTX-treated group ( p < 0.001). ROS levels were decreased in the ASTX group when compared to the 50 mM HG group ( p < 0.001). Scale bar: 80 μ m.

Article Snippet: Human gingival fibroblasts (Accegen Biotechnology, Fairfield, New Jersey, USA, Cat no: ABC-TC3627) were incubated in low glucose (LG) conditions; in a 1 : 1 mixture of Dulbecco's Modified Eagle's Medium/Nutrient Mixture Ham's F-12 (DMEM/F12) containing 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml- μ g/ml penicillin-streptomycin (PS), and 2.5 mg/ml amphotericin B (AMP-B) in a humidified atmosphere of 5% CO 2 at 37°C.

Techniques: Confocal Microscopy

Journal: Communications Biology

Article Title: EP4-induced mitochondrial localization and cell migration mediated by CALML6 in human oral squamous cell carcinoma

doi: 10.1038/s42003-024-06231-4

Figure Lengend Snippet: A Protein expression of EP4 in human gingival fibroblasts cells (HGnF cells) and human OSCC cells (HSC-3). B The EP4 agonist induced a significant increase intracellular Ca 2+ in HSC-3 cells but not HGnF cells. C Representative pictures and quantitative analysis of the scratch assay. These results confirmed that the EP4 agonist promoted the migration of HSC-3 cells but not HGnF cells. D Proposed mechanism of the EP4 signaling-mediated increases in the Ca 2+ concentration and cell migration in HGnF cells and OSCC cells. The schema was created using BioRender. A – C Data are representative of n = 4 independent experiments. A – C Unpaired t test; ns, not significant; ** p < 0.01, *** p < 0.001. Source data and exact p values are provided as a source data file.

Article Snippet: Human gingival fibroblasts (HGnF cells) and human oral keratinocytes were purchased from ScienCell Research Laboratories (CA, USA).

Techniques: Expressing, Wound Healing Assay, Migration, Concentration Assay